Diffusion of organochlorine (OCPs) and cypermethrin pesticides from rohu (Labeo rohita) internal organs to edible tissues during ice storage: a threat to human health.

The migration of organochlorine pesticides (OCPs) and cypermethrin residues from internal organs to edible tissues of ice-held Labeo rohita (rohu) was investigated in this study. The liver (246 µg/kg) had the highest level of ∑OCP residues, followed by the gills (226 µg/kg), intestine (167 µg/kg), and muscle tissue (54 µg/kg). The predominant OCPs in the liver and gut were endosulfan (53-66 µg/kg), endrin (45-53 µg/kg), and dichloro-diphenyl-trichloroethane (DDT; 26-35 µg/kg). The ∑OCP residues in muscle increased to 152 µg/kg when the entire rohu was stored in ice, but they decreased to 129 µg/kg in gill tissues. On days 5 and 9, the total OCPs in the liver increased to 317 µg/kg and 933 µg/kg, respectively. Beyond day 5 of storage, total internal organ disintegration had led to an abnormal increase in OCP residues of liver-like mass. Despite a threefold increase in overall OCP residues by day 9, accumulation of benzene hexachloride (BHC) and heptachlor was sixfold, endrin and DDT were fourfold, aldrin was threefold, and endosulfan and cypermethrin were both twofold. Endosulfan, DDT, endrin, and heptachlor were similarly lost in the gills at a rate of 40%, while aldrin and BHC were also lost at 60 and 30%, respectively. The accumulation of OCP residues in tissues has been attributed to particular types of fatty acid derivatives. The study concluded that while pesticide diffusion to edible tissues can occur during ice storage, the levels observed were well below the allowable limit for endosulfan, endrin, and DDT.

Risk surveillance with spatial distribution of Organochlorine Pesticides (OCPs) from sedimentary samples of Chenab River.

A pioneering study employed a holistic geostatistical approach to predict the spatial variability of a non sampled area in the Chenab River, Pakistan, using kriging interpolation for organochlorine pesticide (OCP)-polluted risk zones. The Present research intended to investigate the carcinogenic and non-carcinogenic human health risks, contamination levels, and spatial variation of OCPs in the Chenab River, Pakistan. The residual OCP content in sediment samples (n = 120) ranged from 0.056 to 32.14 ng/g. DDE and α-HCH were prevalent among all the samples analyzed, with mean concentrations of 15.84 ± 8.02 and 12.45 ± 6.72 ng/g, respectively. The order of magnitude of OCPs in sediment samples was DDTs > α-HCH > chlorothalonil > heptachlor > endosulfan > aldrin > dieldrin. The findings of the single (SPI) and Nemerow (Nel) pollution index of α-HCH, heptachlor, and aldrin depicted the Chenab River as a serious pollution risk zone. The outcomes of the Pearson correlation coefficient analysis represent the positive correlation among all OCPs, revealing the common origin. Distribution trends showed substantially higher (p < 0.05) contents of analyzed OCPs along the downstream zone. With regards to USEPA human health hazard assessment model, the estimated non-carcinogenic (ΣHI) and non-carcinogenic (ΣTCR) risk ranged from 1.1 × 10-5 to 1.0 × 10-1, 4.0 × 10-8 to 3.2 × 10-4 respectively. TCR >10-4 illustrated a substantial cancer health risk posed by α-HCH, heptachlor, aldrin, and dieldrin in the downstream zone. We recommend the urgent cessation of the ongoing discharge of OCPs into the Chenab River, which needs to be highlighted owing to the significant cancer risk to public health to ensure the good health and wellbeings.

Water quality, human health risk, and pesticides accumulation in African catfish and Nile tilapia from the Kitchener Drain-Egypt.

Pesticides are toxic and could negatively impact humans and the ecosystem. The Kitchener Drain is among the longest drains in Egypt and carries a wide range of wastewater from the agriculture sector, which contains pesticides and may pollute the ecosystem. Thus, water quality, human health risk, and pesticide accumulation in African catfish and Nile tilapia from the Kitchener Drain-Egypt. The water and fish samples were collected from Kitchener Drain in Kafr Elsheikh Governorate, Egypt, during the four seasons. The results indicated that heptachlor and diazinon were undetected during the four seasons. However, endosulfan, chlorpyrifos, and dicofol were detected in winter and autumn. Only p,p'-DDT was detected during spring. Endosulfan, heptachlor, and aldrin were detected in Nile tilapia during winter. Only heptachlor and aldrin were detected during spring. Endosulfan, heptachlor, dicofol, p,p'-DDT, chlorpyrifos, and diazinon were detected in the autumn season. In summer, dicofol and p,p'-DDT were detected, while endosulfan, heptachlor p,p'-DDT, aldrin, chlorpyrifos, and diazinon were not detected. In African catfish, endosulfan, heptachlor, dicofol, and p,p'-DDT were detected during winter, while chlorpyrifos, aldrin, and chlorpyrifos, aldrin, and diazinon were not detected. In the spring season, endosulfan, heptachlor, and aldrin were detected. Endosulfan, heptachlor, dicofol, p,p'-DDT, aldrin, chlorpyrifos, and diazinon were detected in the autumn season. Similarly, in the summer season, endosulfan, heptachlor, dicofol, p,p'-DDT, aldrin, chlorpyrifos, and diazinon were detected. The sequence of estimated daily intake (EDI) in Nile tilapia during the four seasons is heptachlor > endosulfan > dicofol > p,p'-DDT > aldrin > diazinon > chlorpyrifos. The sequence of EDI in African catfish during the four seasons is endosulfan > p,p'-DDT > heptachlor > aldrin > dicofol > diazinon > chlorpyrifos. In conclusion, the results confirmed the absence of a hazard index for consuming Nile tilapia and African catfish collected from the Kitchener drain.

Correlation Between Toxic Elements and Pesticide Residues in Medicinal Herbs Available in Pharmaceutical Market.

The use of medicinal plants for self-medication of minor health conditions has become a widespread practice in contemporary society. Few consumes, however, question the contamination of these products with toxic factors resulting from the planet's increasingly polluted environment. This paper presents the levels of five toxic elements (As, Cr, Pb, Cd, and Hg) and nine organochlorine pesticides (hexachlorobenzene (HCB), lindane, heptachor, aldrin, dieldrin, endrin, p,p'DDE, p,p'DDD, and p,p'DDT) in 14 brands of regularly consumed medicinal products in Romania. The toxic elements content was determined using energy-dispersive X-ray fluorescence (EDXRF) technique, and organochlorine pesticide residues (OPCs) were quantified using gas-chromatographic method, equipped with electron capture detector (GC-ECD). The results show that in the case of Cr, Cd, and Hg, the concentrations exceeded the limit values established by World Health Organisation (WHO) for raw herbal material. The higher level of OPCs (such as p,p'DDD, p,p'DDT, aldrin, and dieldrin) was found in the samples of Hypericum perforatum-St. John's wort, Crataegus monogyna-hawthorn, and Epilobium parviflorum-hoary willowherb. The correlations between the content of toxic elements and pesticides were determined by statistical analysis. Hierarchical clustering technique was used to detect natural grouping between the toxic elements and pesticides. For herb samples, four clusters were identified, the strongest correlated cluster consisting of Pb, HCB, Cr, and Hg. A further analysis within this cluster suggested that Cr levels are statistically different from the rest of the elements.

A national-scale distribution of organochlorine pesticides (OCPs) in cropland soils and major types of food crops in China: Co-occurrence and associated risks.

Organochlorine pesticides (OCPs) are persistent organic pollutants (POPs) that resist degradation in the environment. OCPs remain detectable in cropland systems in China. However, spatial distribution of OCPs across China and associated ecological and health risks, as well as the relationship between levels of OCPs in cropland soils and crops, remain to be elucidated. To fill these gaps, we conducted a national-scale characterization of 19 individual OCPs in cropland soils and food crops including cereals and legumes in China, which were on-spot sampled simultaneously. Sparse canonical correlation analysis was employed to investigate the co-occurrence of OCPs in cropland soils and corresponding food crops. The ecological soil screening levels and risk quotient method were adopted for ecological and health risk assessment, respectively. Dichlorodiphenyltrichloroethanes (DDTs) were dominant in cropland systems, with its levels ranging up to 337 and 22.8 µg/kg in cropland soils and food crops, respectively. The mean ∑OCP levels in cropland soils varied from below the limit of detection to 337 µg/kg. Peanuts were the most contaminated crop, in which endosulfans and hexachlorobenzene (HCB) were co-occurrent with those in cropland soils (correlation coefficient R = 0.999 and 0.947, respectively). Besides, lindane and ß-endosulfan in rice were co-occurrent with those in cropland soils (R = 0.810 and 0.868, respectively). The componential ratio analysis indicated fresh inputs of technical DDT, lindane, chlordane, endosulfan, HCB and aldrin. Among these pesticides, ecological impacts of DDTs, lindane, aldrin and ß-endosulfan could be expected. Human health risk assessment suggested that daily consumption of the OCP-contaminated food crops raises a health concern especially for male teens. It is concluded that OCPs remain present in cropland systems in China at levels that raise a concern for both environment and human health.

Organochlorine pesticides in therapeutic teas and human health risk assessment.

This study evaluated commercially available therapeutic teas for their organochlorine pesticides (OCPs) residue levels to estimate the potential health risks associated with their prolonged consumption. Tea samples that were produced for therapeutic uses were obtained commercially. Organochlorine pesticide residues were extracted from the samples using the modified QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) method and analysed using Gas Chromatography with Electron Capture Detector (GC-ECD). Results showed that the tea samples contained a large number of OCPs residue at levels that were significantly higher than the maximum residue limits (MRLs) set by the European Union (p > 0.05). Aldrin was detected in all samples, methoxychlor, and p,p'-DDT were detected in ≥88% of the samples while γ-HCH, δ-HCH, endosulfan II, and endosulfan sulphate were detected in ≥69% of the samples. Health risk assessment indicated that the highest risk of cancer was associated with aldrin.

Agricultural pesticide residues in water from a karstic aquifer in Yucatan, Mexico, pose a risk to children's health.

Yucatan is a region with a high impact of water contamination since it has a karst type soil favoring contaminants entry into the phreatic level, the only source of freshwater in the area. However, no studies report pesticides in water for human consumption or the risk it represents. The objective of this study was to detect and measure pesticide concentrations in domestic tap water to estimate the risk (carcinogenic and non-carcinogenic) to health. A non-probabilistic sampling was applied of 48 tap water sources, and then pesticide detection with solid-phase extraction gas chromatography coupled to the electron capture and flame photometric detectors allowed the estimation of risk through hazard ratios. The present results suggest that aldrin, heptachlor, and ß-BHC residues in domestic tap water from Ticul, Yucatan, pose a risk to children's health, particularly for potential carcinogenic risks.

Organochlorine pesticide residues in Uganda's honey as a bioindicator of environmental contamination and reproductive health implications to consumers.

Honey has multifaceted nutritional and medicinal values; however, its quality is hinged on the floral origin of the nectar. Taking advantage of the large areas that they cover; honeybees are often used as bioindicators of environmental contamination. The focus of the present paper was to examine the quality of honey from within the vicinity of an abandoned pesticide store in Masindi District in western Uganda. Surficial soils (<20 cm depths) and honey samples were collected from within the vicinity of the abandoned pesticide store and analysed for organochlorine pesticide (OCP) residues using gas chromatograph coupled to an electron capture detector (GC-ECD). The mean level of ∑DDTs in all the soil samples was 503.6 µg/kg dry weight (d.w). ∑DDTs contributed 92.2% to the ∑OCPs contamination loads in the soil samples, and others (lindane, aldrin, dieldrin, and endosulfans) contributed only 7.8%. Ratio (p, p'-DDE+p, p'-DDD)/p, p'-DDT of 1.54 suggested historical DDT input in the area. In all the honey samples, the mean level of ∑DDTs was 20.9 µg/kg. ∑DDTs contributed 43.3% to ∑OCPs contamination loads in the honey samples, followed by lindane (29.8%), endosulfans (23.6%) and dieldrin (3.2%), with corresponding mean levels of 14.4, 11.4 and 1.55 µg/kg, respectively. Reproductive risk assessment was done based on the hazard quotient (HQ) and hazard index (HI) procedure. In our study, the calculated HIs for adults (102.38), and children (90.33) suggested high potential health risks to the honey consumers. Lindane, endosulfan and p, p'-DDD detected in the honey samples at levels exceeding the acute reference dose (ARfD) are known risk factors for spontaneous abortion, reduced implantation, menstrual cycle shortening, impaired semen quality, and prostate cancer in exposed individuals and experimental animal models.

Comprehensive assessment of unutilized and obsolete pesticides impact on genetic status and health of population of Almaty region.

The group of persistent organic pollutants (POPs) are particularly dangerous for the environment and by consequence for human health because of the risk to be transmitted in the food chain. Among them, the urgent problem of obsolete and forbidden organochlorinated pesticides (OCPs) needs a rigorous management in many countries, including Kazakhstan. The aim of our study was to evaluate the effect of pesticides content in food products on the genetic status and health of the population living on the contaminated areas near destroyed warehouses for OCPs (4 villages of Talgar district and 1 control site, Almaty region). The food products sampled in Taukaraturyk (control site), and in 4 villages where non-utilized obsolete pesticides were discovered: Beskainar, Kyzylkairat, Amangeldy, and Belbulak. The contents of 24 pesticides in food products from plant (apples, pears, tomatoes, cucumbers, sweet peppers) and animal (beef meat, cow milk, honey) origin, that grown in places of localization of non-utilized OCPs, were determined, sometimes in high and unacceptably high concentrations (before 2500 times over MRL). In pears, the pesticides content (especially DDT, γ-HCH, ß-HCH, endosulfan, and aldrin pesticide group), was higher than in other fruits. Among vegetables, the highest levels of all groups of pesticide were found in cucumbers. Beef meat samples demonstrated increased contents of ß-HCH, γ-HCH, endrin and dieldrin. In cow milk samples only the high concentration of dieldrin was found. The content of pesticides in meat was 4-5 times higher than in milk. The medical examinations, carried out among the cohorts living around the polluted by pesticides territories and control cohort from ecologically favorable village, showed that there were more individuals with high and middle levels of somatic health in the control group than in groups exposed to OCPs. The long-term effect of the pesticide contamination of the environment on genetic status of the population was assessed by chromosomal aberration (CA) frequencies. The highest level of chromosomal aberrations was identified for the examined residents of Kyzylkairat (41%) and Belbulak (38%), a high level in Amangeldy (12%), and middle level in Beskainar (6.5%). The association between the CA frequency, health status and the pesticides contents in food were assessed by a Spearman rank correlation. The low indicators of somatic health status were strictly associated with high levels of CA, and good health status indicates that the CA rates did not exceed the spontaneous level of mutagenesis. The strongest correlation was shown between high levels of chromosomal aberrations and the content of different pesticides in pears (Cr = 0.979-0.467), tomatoes (Cr = 0.877-0.476), cucumbers (Cr = 0.975-0.553) and meat (Cr = 0.839-0.368). The obtained results highlight the need to improve health protection by increasing the public awareness to the security of the storage of obsolete OCPs in order to strengthen food safety by efficient control services.

Quantitative analysis of pesticide residues in tea by gas chromatography-tandem mass spectrometry with atmospheric pressure chemical ionization.

In this study, gas chromatography-tandem mass spectrometry (GC-MS/MS) using an atmospheric pressure chemical ionization (APCI) source was applied for the quantitative analysis of pesticide residues in tea. To determine the optimum ionization conditions for multiresidue analysis, the full-scan mass spectra and peak intensities of pesticides were compared in the presence and absence of water as a modifier. When water was added as a modifier in the ion source, most of the target compounds formed [M+H]+ ions and exhibited enhanced intensities. However, compounds consisting of only carbon, hydrogen, and chlorine, such as aldrin, γ-hexachlorocyclohexane, and p,p'-dichlorodiphenyldichloroethane, typically formed M+· or fragment ions, whose intensities were significantly decreased by the addition of water. GC-MS/MS methods using APCI (without modifier addition) and electron ionization (EI) were validated for 16 pesticides in tea at spiking levels of 0.01 and 0.1 mg/kg. Unlike EI, signal suppression was observed for most compounds at a spiking level of 0.01 mg/kg using APCI; however, dilution of the samples minimized this effect. Using APCI, the trueness of the target compounds ranged from 77% to 121% at both spiking levels, except for pyrethrins owing to matrix effects, with relative standard deviations of less than 14%. For most compounds, these results were comparable with those obtained using EI. However, because the use of APCI limited fragmentation, this ionization technique offered significantly higher sensitivity and specificity than EI. Using APCI, linear calibration curves with coefficients of determination greater than 0.998 were obtained in the range of 0.0005-0.5 µg/mL for all compounds. These findings indicated that GC-MS/MS with APCI is applicable for the routine monitoring of pesticide residues, even in complex samples such as tea.

Pesticides (Hexachlorocyclohexane, Aldrin, and Malathion) Residues in Home-Grown Eggs: Prevalence, Distribution, and Effect of Storage and Heat Treatments.

The abuse of pesticides in home gardens may lead to contamination of home-grown eggs. This study aimed to determine the prevalence of hexachlorocyclohexane (HCH), aldrin, and malathion pesticides residues in egg white and egg yolk of home-grown eggs in Jordan; and the effect of refrigerated storage and heat treatment (boiling and frying) on residues level. High-performance liquid chromatography was used to detect pesticide residues in egg samples (n = 200) obtained from households that raise laying hens in Jordan. About 96% of the tested eggs showed pesticide residues. None of the tested egg samples showed residual level above maximum residue limit (MRL 0.02 mg/kg) for HCH, whereas 33% and 44% of samples were above MRL for aldrin and malathion, respectively. All studied pesticide residues were detected from both egg yolk and egg white. The concentrations of malathion and aldrin (0.075 and 0.067 mg/kg) in egg yolk were higher than those (0.049 and 0.058 mg/kg) in egg white samples. Pesticide residue levels were quite stable during refrigeration storage whereas heat treatment (boiling at 100 °C and frying at 160 °C) significantly reduced contamination levels to values below MRL. The high level of pesticide residues in home-grown egg in Jordan may reflect the improper use of pesticides in home gardens and thus exposing the environment to unwanted pollution and the risk they may pose on human health. PRACTICAL APPLICATION: Home-grown eggs could be exposed to pesticides more than commercial eggs as free-range hens interact directly with the environment and ingest soil or materials on/in the soil that might be contaminated with pesticides used in home gardens or farms. Exploring pesticides residues in home-grown eggs and effect of refrigerated storage and heat treatment (boiling and frying) on residue levels would be useful to consumers and health authorities.

Dietary exposure and risk assessment of organochlorine pesticide residues in rural communities living within catchment areas of iSimangaliso World Heritage Site, South Africa.

Dietary intake of contaminated food is a major route of human exposure to organochlorine pesticides (OCPs). Despite the prevalence of OCP usage in southern Africa for several decades, their impact on socioeconomically vulnerable communities remains largely overlooked. We investigated the accumulation of OCPs in crops commonly cultivated by rural communities surrounding iSimangaliso Wetland Park, South Africa. All samples analysed were found to be contaminated, with total OCP concentrations ranging between 190 and 240 ng g-1 ww. Elevated levels of OCP residues were detected in all products, with the majority of samples exceeding European Commission's maximum residue limits (MRLs). We combine these results with local OCP fish tissue estimates to assess potential dietary risks. Cumulative risk assessment indicated potential non-cancer risks associated with heptachlor epoxide, while a cancer risk as high as 1 in 10 was found to be associated with aldrin and dieldrin. This far exceeds USEPA guidelines and indicates that OCP residues in crops and fish from the study area pose a high risk to human health. The results of this study call for further attention to be given to the health implications of continued OCP use not only within the study area but also in Southern Africa in general.

Risk Of Human Dietary Exposure To Organochlorine Pesticide Residues In Fruits From Ghana.

The objectives of this present study were to assess the level of organochlorine pesticide (OCP) residues in fruits and to determine the potential health risks associated with the exposure to these pesticides. A total of 120 fruits (watermelon, pineapple, and banana) were collected from five communities and a local market within the Mampong Municipality and analyzed for organochlorine pesticide residues. The results showed that the concentrations ranged from not detectable (ND)-48.22 ng/g for DDTs, ND-19.03 ng/g for HCHs, ND-4.10 ng/g for CHLs, ND-22.84 ng/g for Aldrin, and ND-11.53 ng/g for other OCPs. Levels of methoxychlor, Aldrin and gamma-hexachlorocyclohexane (HCH) exceeded the maximum residue limits in watermelon. Estimated health risk revealed that Aldrin in watermelon could pose potential toxicity to the consumer. Estimated average daily intake for Aldrin was above the acceptable average daily intake.

Occurrence of organochlorine pesticides in a tropical lake basin.

This study investigates the presence and distribution of organochlorine pesticides in streams and the lake in the Sembrong Lake Basin in Malaysia. The catchment of Sembrong Lake has been converted to agricultural areas over the past 30 years, with oil palm plantations and modern agricultural farming being the main land use. Surface water samples were collected from eight sites comprising the stream and lake and analysed for 19 organochlorine pesticides (OCPs). In situ measurement of temperature, dissolved oxygen, pH and conductivity were also undertaken at each site. Aldrin, endrin, δ-BHC, 4,4-DDT, methoxychlor and endosulfan were the main OCPs detected in the lake basin. The total OCP concentration ranged between 5.42 and 349.2 ng/L. The most frequently detected OCPs were δ-BHC, heptachlor and aldrin. The maximum values detected were 23.0, 43.2 and 50.4 ng/L respectively. The highest concentration of OCPs was attributed to 4,4-DDT, but such high residue was rare and only detected once. Other OCP residues were low. Significant differences in the mean values were observed between lake and stream for dichlorodiphenyldichloroethylene (DDE) and α-endosulfan concentration (p < 0.05). The highest endosulfan, endrin and methoxychlor residues were found in lake sites, while the highest 4,4,-DDT residues were observed for the river sites. Temporal variation of OCP residues was observed for heptachlor and ß-endosulfan. The highest concentrations of pesticide residues were found in October. DDX and α/É£ ratios indicate possible fresh inputs of the OCP pesticide in the basin.

Pesticide residues in raw and processed maize grains and flour from selected areas in Dar es Salaam and Ruvuma, Tanzania.

This study investigated the levels of pesticide residues in maize grains and flour and the effects of processing methods on their levels in maize products in samples collected in Dar es Salaam and Ruvuma regions. Analysis of cleaned-up extracts was done using gas chromatography-mass spectrometry (GC-MS). Twelve pesticides were detected in maize grains and their highest concentrations were up to 676.1, 11200 and 14 µg/kg for organochlorines, organophosphorous and pyrethroid pesticides, respectively. In maize flour, eight pesticides were detected and the concentrations for organochlorines, organophosphorous and pyrethroid pesticides were up to 333.3, 2220 and 2 µg/kg, respectively. Only dieldrin was detected in cooked samples at a concentration of 2 µg/kg. The concentrations of p,p'-DDD, aldrin, dieldrin, chlorpyrifos and pirimiphos methyl in some grains and flour samples exceeded the maximum residue limit (MRLs). The findings indicate risks and concerns for public health. Processing methods were found to cause transformation and reduction of the pesticides.

Organochlorine Pesticides Residues in Human Breast Milk from the Middle Governorates in Jordan in 2013/2014.

One hundred samples of mother breast milk were gathered from six middle governorates and districts in Jordan in 2013/2014 to monitor Organochlorine pesticides pollutants. The results showed clearly that banned organochlorine pesticides are still detected in the monitored samples in low concentration despite banning of these persistent pollutants in Jordan since 36 years ago. However, the results indicated that 1% of the contaminated samples contained ß-HCH, 5% γ-HCH, 3% p,p'-DDD, 2% heptachlor, 45% p,p'-DDE and 3% p,p'-DDT. In addition, these monitored samples had no residues of aldrin, dieldrin, α-endosulfan, ß-endosulfan, HCB, o,p'-DD, o,p'-DDT and o,p'-DDE. In conclusion, there was a decline in the residues of Organochlorine pesticides, particularly DDT group members.

Pesticide survey in water and suspended solids from the Uruguay River Basin, Argentina.

The Uruguay River is receptor of pollutants, such as pesticides, from agriculture activities along its course. The present study reports concentration levels of organochlorinate, organophosphorus, and other pesticides in water and suspended solids in nine sampling sites of the Uruguay River. Data analyses included principal component analysis (PCA) to assess differences between sampling sites contamination. Most of the tested pesticides were ubiquitous due to the widely use in the chemical control of pests implemented in the region. Detected concentrations of aldrin, chlordane, dieldrin, endrin, heptachlor epoxide, lindane, 4,4'-DDT, endosulfan, chlorpyrifos, diazinon, methyl-parathion, and malathion were found to be over regional and international concentration level guidelines, according to the European Union, the US Environmental Protection Agency, or the Argentinean Secretariat of Environment and Sustainable Development. For this reason, future studies in Uruguay River Basin are needed.

Pesticide exposure in the local community of Vehari District in Pakistan: An assessment of knowledge and residues in human blood.

The present study is based on cross-sectional data collected from rural and market areas of Vehari District in Pakistan to assess public awareness of pesticide risks and determine the levels of exposure to organochlorine pesticides (OCPs) in the local community. Blood samples were collected from 56 volunteer donors (VDs) including children, female workers, farm workers involved in pesticide business, farm workers involved in pesticide spraying activities, and people who were living away from agricultural fields. Blood analysis showed that VDs who were involved in spraying activities had significantly higher levels of OCP residues in their blood samples than VDs from the other groups, with mean concentrations of 1.13, 0.92, 0.68 and 1.96ngmL-1 for pp-DDT, aldrin, dieldrin, and endosulfan, respectively. However, VDs who were living away from agricultural fields had significantly lower levels of pesticide residues in their blood samples, with mean concentrations of 0.30, 0.19, 0.14 and 0.41ngmL-1 for pp-DDT, aldrin, dieldrin, and endosulfan, respectively. A survey of 179 volunteer respondents (VRs) showed that a significant proportion of the VRs had little knowledge of using the recommended amounts of pesticides (65.9%). Furthermore, the majority of the VRs was found using limited protective measures during pesticide use (62.6%) and was practising unsafe storage of pesticides (87.7%). In addition, most farm workers (88.8%) reported an increasing trend in pesticide use in their farms each year. Knowledge of pesticide risks on human health increased with formal education and training. Poor knowledge regarding pesticide risks and handling among inhabitants of Vehari District contribute to high exposure levels to OCPs, particularly among farm workers. Findings are useful for policy formulation aimed at reduction of pesticide exposure in Pakistan.

Distribution and health risk assessment of organochlorine pesticides (OCPs) residue in edible cattle tissues from northeastern part of Egypt: High accumulation level of OCPs in tongue.

Food consumption is an important route of human exposure to organochlorine pesticides (OCPs). In order to assess the potential human health risks associated with OCPs, edible cattle tissues (liver, kidney and tongue) were collected from three slaughter houses in Mansoura, Zagazig and Ismailia cities, Egypt. Levels of 22 OCPs such as hexachlorocyclohexanes (HCHs), dichlorodiphenyltrichloroethanes (DDTs), aldrin, dieldrin and endrin (Drins), chlordanes (CHLs), heptachlors (HPTs) and hexachlorobenzene (HCB) residues were investigated. Among the investigated OCPs, HCHs represented the most dominant group with high proportions of γ-HCH isomer (53-91% of total HCHs). Mansoura city had the highest OCPs contamination load ranged from 0.1 to 2827 ng g(-1) lw (lipid weight). Surprisingly, tongue samples collected from Mansoura showed the highest concentration of HCHs (448 ng g(-1) lw) in comparison to liver (152 ng g(-1) lw) and kidney (266 ng g(-1) lw). Generally, contamination pattern of OCPs was in the order of HCHs > Drins > CHLs > DDTs ≅ HCB and HPTs. Estimated daily intakes (EDIs) through dietary consumption of cattle tissues were lower than the recommended acceptable daily intakes (ADIs) established by FAO/WHO. However, the hazard ratios (HRs) based on cancer risk were greater than 1.0 for HCHs based on the average and 95th centile concentrations, indicating carcinogenic effects to consumers through cattle tissues consumption.

Distribution and risk assessment of suspected endocrine-disrupting pesticides in creek water of Mumbai, India.

The present study deals with the investigation of existing pollution levels and potential ecological risk assessment of endocrine-disrupting organochlorine and organophosphorous pesticide residues in the Vasai Creek water near Mumbai. The average concentration of α- and ß-endosulfan (137.75 ng·L(-1)) exceeds the chronic criteria level of α- and ß-endosulfan (6.5 ng·L(-1)) set by US EPA for freshwater aquatic organisms. The concentration levels of aldrin (75.31 ng·L(-1)), dieldrin (71.19 ng·L(-1)) and endrin (76.60 ng·L(-1)) was found to exceed the respective criteria levels of <0.13, 65.1, and 61 ng·L(-1) as set by US EPA for protection of freshwater aquatic organisms. In addition, the level of chlorpyrifos (208.77 ng·L(-1)) exceeds the recommended concentration value of <35 ng·L(-1) set by Ministry of Environment of British Colombia. The results of our study give an indication of probable ecotoxicological risk to the marine breeding organisms of creek.